Effects of 6-Shogoal on the Apoptosis of Human Colorectal Cancer Cells and the Expression of Bax, BCL2, Caspase3 and pARp1
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Abstract:
In this study, HCT116 and HT29 were directly induced by 6-shogoal through in vitro cell experiment to investigate the effects of 6-shogoal on the apoptosis of human colorectal cancer cells and the expression of associated apoptotic proteins (Bax, BCL2, Caspase3 andPARP1). The changes of cell morphology with the treatment of 6-shogoal were observed by inverted phase contrast microscope. The inhibition rate of HCT116 and HT29 was detected by CCK8 and the cell proliferation curves were drawn.The apoptosis in human HCT116 and HT29 were detected by Annexin V-FITC/PI flow cytometr and the expression of related proteins (Bax, BCL2, Caspase3 and pARp1) was analyzed using western-blot). Compared with the model group, 6-shogoal could inhibit the apoptosis of HCT116 and HT29 in a concentration dependent manner (p<0.05), which also could enhance the expression of Bax, Caspase3 and PARP1 inhibit the expression of BCL2 and increase the ratio of Bax/BCL2 (p<0.05). Therefore, the apoptosis of HCT116 and HT29induced by 6-shogoalmay be related to the activation of Bax, Caspase3, PARP1 expression and the increase of the Bax/BCL2 ratio. This finding can provide references for the clinical treatment and prevention of colorectal cancer.
