Effect of BPS on the Differentiation of 3T3-L1 Preadipocytes and Its Mechanism
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Abstract:
Bisphenol A (BPA) is restricted for its endocrine disruption and adverse health effects on the human body, so that bisphenol S (BPS) is widely used as a substitute n a variety of food packaging materials. The security of BPS has not been fully studied before its large-scale application.. In this study, 3T3-L1 preadipocytes were used as an in vitro model to investigate the effect of BPS on the differentiation of 3T3-L1 preadipocytes and its mechanism, to evaluate the safety of BPS and provide reference for the prevention of chronic metabolic diseases such as obesity. The results showed that BPS could promote the growth of 3T3-L1 preadipocytes and could significantly inhibit the proliferation of cells when the concentration was over 400 μmol/L. BPS could significantly induce the accumulation of lipid in the differentiated 3T3-L1 cells which showed a non-dose-dependent relationship. Compared with the model group, BPA up-regulated the expressions of PPARγ, C/EBR and aP2 genes, whereas BPS only seems to up-regulate the expression of PPARγ. In addition, BPS and BPA could significantly decrease the expression of GLUT4 gene (p<0.01). In conclusion, the pathways of BPS and BPA acting on adipocytes differentiation were not exactly the same, which might be accomplished under the combined action of various transcription factors.
