Abstract:Thin Layer Chromatography (TLC) was used to identify the differences of main active components between Acanthopanax sentcosus (Ruper.et Maxim.) Harms (ASR) and Acanthopanax sessiliflorus (AS), which were obtained from Jilin, Heilongjiang and Liaoning. The content of Eleutheroside B, Eleutheroside E and Isofraxidin were determined by High Performance Liquid Chromatography (HPLC). The results showed that the Isofraxidin characteristic band of ASR displayed on TLC chromatogram was obvious using a ultraviolet lamp (365 nm). While the Isofraxidin characteristic band of AS was not obvious, it still existed on TLC chromatogram. Consequently, the identification method could improve quantitative indicator that was to give a definite conclusion. The HPLC results showed that the content of three functional components in ASR samples were high, and the average content of Eleutheroside B and Isofraxidin of ASR samples were higher than that of AS samples. The content of Eleutheroside B of several AS samples was nearly 0.1%, which was higher than the level regulated by Chinese Pharmacopoeia (0.05%). The content standard of Eleutheroside B could be improved for further study to avoid the adulterant of ASR.
