Determination of Forchlorfenuron and Its Metabolites in Kiwifruit Using QuEChERS-LC-MS/MS
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Abstract:
Forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea) is a plant growth regulator, which mainly be used to increase the size and weight of kiwifruit. The standards of forchlorfenuron metabolites were obtained by chemosynthesis and forchlorfenuron and its metabolites (4-hydroxyphenyl- forchlorfenuron, 3-hydroxyphenyl-forchlorfenuron, forchlorfenuron-4-O-β-D-glucoside and forchlorfenuron-3-O-β-D- glucoside) in kiwifruit were simultaneously determined by LC-MS/MS with ESI+ under the mode of multiple reaction monitoring (MRM) in this study. The pre-treatment of sample was improved by optimizing PSA addition with acetonitrile as extraction solvent for extraction of clopidourea residue. The results showed that forchlorfenuron and its metabolites had a good linear correlation within a certain concentration range, the relative standard deviations were below 7.1% with a good repeatability, the limit of detection (LOD) was 1~3 μg/L with a limit of quantification (LOQ) of 5~10 μg/L, and recoveris were between 87% and 108% with the PSA addition of 400 mg/15 mL. Therefore, QuEChERS-LC-MS/MS was sensitive and accurate, which could be used for simultaneous determination of forchlorfenuron and its metabolites in kiwifruit.
