Determination of the 56 Pesticide Residues in Vegetables by QuEChERS-ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
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Abstract:
A new method for analysis of 56 pesticide residues in vegetables using modified QuEChERS-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established to solve the technical difficulties of complex components, interference and low concentration. Vegetable samples were pretreated bymodified QuEChERS, which was extracted with acetonitrile containing 1% (V/V) acetic acid, and then salted out with sodium chloride and anhydrous sodium. purified with primary secondary amine, samples was analyzed by HPLC-MS/MS. The extract was separated on a C18 column (4.6×100 mm, 2.6 μm) using acetonitrile and aqueous solution as the mobile phase (containing 5 mM ammonium acetate and 1% (V/V) acetic acid), ionized in electrospray ion (ESI+) mode and then analyzed in multiple-reaction monitoring (MRM) mode. The results showed that the 56 pesticide residues showed good linear correlation in the range of 2.0 μg/L to 100 μg/L with the correlation coefficients over 0.99. The limits of detection were less than 0.01 mg/kg, and the recoveries were ranged of 65.8%~114.0% at three spiked levels in vegetables with the relative standard deviations of0.1%~14.5%. This proposed method is rapid, simple, low-cost and effective, which can be applied to rapid screening and analysis of pesticide residues in vegetables for the risk assessments of quality and safety.
