Staphylococcus aureus Drug Resistance of Dairy Cow Mastitis in Tianjin Region
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Abstract:
To understand the pathogenicity of Staphylococcus aureus in dairy cow mastitis and the sensitivity to antibiotics in Tianjin area. Mannitol salt medium and high lyophilized rabbit plasma were used for isolation and identification of 50 samples that were collected from seven cattle farms in Tianjin to provide scientific references for treatment of dairy cow mastitis and prevention of veterinary drug residues, and the susceptibility tests were performed by disk diffusion method. 29 Staphylococcus aureus and 4 other pathogenic Staphylococci were isolated and identified. Drug resistance of isolated Staphylococcus aureus were analyzed with 8 categories of 29 species commonly used antibiotics. Staphylococcus aureus isolates were amplified by PCR of the drug resistance gene and detected by agarose gel electrophoresis. The result showed that 29 isolates of Staphylococcus aureus had different resistance to 8 categories of 29 species antibiotics. The drug resistance rates to macrolides and lincosamides were generally high, which were 77.01% and 89.66% in average, and the drug resistance rates to β-lactams, quinolones and aminoglycoside were all 50% below. The isolates had the complete resistance (100%) to penicillin G, Acetyl-Lo and lincomycin, and the 20%~40% resistance rates to cefoperazone, cefotaxime, amoxicillin, ampicillin, enrofloxacin, neomycin, spectinomycin. Analysis of the drug resistance gene showed that the positive rate of drug resistance gene was generally less than that of phenotypic rate of drug resistance gene.
