Optimization of Ultrasonic-Assisted Extraction of Pectin from Premna microphylla Leaves and Analysis of Product Properties
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Abstract:
Premna microphylla leaves were wildly existed in Qinba Mountain with high pectin content and low degree of esterification. In order to make full use of Premna microphylla leaves, the effects of different solvents-pH, extraction temperature and ultrasonic power on the yield of pectin extraction were investigated. Response surface methodology was employed to optimize the pectin extraction conditions from Premna microphylla leaves. The mathematical model was established according to Box-Behnken central composite design. Besides, the fractionally precipitates with ethanol concentration of 30% and 50% respectively were produced from the optimized extraction products, and their physicochemical properties and infrared spectrum were studied. The results showed that the fitted quadratic regression model was valid. The optimum extraction parameters were pH 1.9, extraction temperature 70 ℃, and ultrasonic power 630 W. Under these conditions, the maximal rate of pectin extraction yield was 23.16%, which was close to the predicted value with 23.20%. The pH and ash value of 30% and 50% fractionally precipitates were in accordance with the requirement of QB 2484-2000 and their typical characteristics were also shown by infrared spectrum.
