Optimization of Extraction Technology of Total Flavonoids and Puerarin in Rdix Puerariae and their Enhanced Antioxidant Capacity in Colitis Rats
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Abstract:
Based on the traditional ethanol heat reflux method to extract puerarin and flavone, the quadratic-orthogonal-rotation -combination design was used to optimize the extraction of total flavonoids and puerarin from Rdix Puerariae. Regression models were established for the yield of total flavonoid (y1) and the yield of puerarin (y2) with the other four test factors (including extraction temperature (x1), reflux time (x2), ethanol concentration (x3) and ratio of material to liquid (x4), respectively. The regression model showed that, the most important two factors that affected the total flavonoids yield and puerarin yield from Radix Puerariae were: extraction temperature (x1) and ethanol concentration (x3). The optimal technical conditions were: extracting at 85 ℃ for 55 min, reflux time 55 min, ethanol concentration of 30%, solid-to-liquid ratio of 1:17.5. Under these conditions, the extraction rate of total flavone from Radix Puerariae was 41.07 mg/g, the extraction of puerarin rate could be 16.50 mg/g, and the optimized extraction technology of Radix Puerariae was stable and feasible. The results showed that the Rdix Puerariae extractive had a better effective in recovering serum catalase (CAT), Cu/Zn superoxide dismutase (SOD), and phospholipid hydroperoxide glutathione peroxidase (GSH-px) levels as well as antioxidant capacity in colitis rats as compared with pure puerarin.
