Does-effect Relationship of Active Ingredients from Supernatant Water of Dendrobium devonianum Paxt.
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Abstract:
In order to utilize the resource of Dendrobium devonianu Paxt., the content of alkaloids, polyphenols, flavonoids and polysaccharides in the supernatant of Dendrobium byproducts were determined after the polysaccharides were extracted by traditional method and enzyme assisted extraction method. The antioxidant and hypoglycemic activities of the supernatant were characterized by the DPPH free radical scavenging rate, the hydroxyl radical scavenging rate, the total reducing capacity and α-amylase inhibition capacity. The results showed that alkaloids, polyphenols and flavonoids and polysaccharides content as well as biological activity in supernatant of Dendrobium byproducts after the polysaccharides were by pectinase assisted extraction were much higher than those in traditional method. The content of alkaloid, polyphenols, flavonoids and polysaccharide in supernatant of Dendrobium, treated with 7.5 U pectinase has the highest material content were 0.1971, 0.6873, 28.4598 and 7.0500 mg/g, respectively; The supernatant of Dendrobium devonianu Paxt treated with 12.5 U pectinase have the strongest antioxidant capacity, the DPPH free radicals’ and hydroxyl lradicals IC50 value were1.25 and 4.1 mg/mL, respectively. After analysis the relationship of does-effect, the content of polyphenols, flavonoids in the supernatant and the total water extract extracted by the traditional method were significantly correlated with the functional indexes, there was no significant correlation between the supernatant and the functional indexes after enzyme treatment.
