Analysis of Antibiotic Resistance and Related Genes of Salmonella enteritidis and Salmonella havana
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Abstract:
The drug susceptibility and the related resistance genes of 17 strains of salmonella enteritidis and 11 strains of salmonella Havana had been studied in detection by Xinjiang Urumqi part of the farmer's market, in order to ensure food safety, better understanding the development of drug resistance and the ways to spread. The drug sensitivity of Salmonella was evaluated by agar dilution method. In addition, PCR and gene sequencing were used to detect the presence of mutations in the quinolone resistancedetermining region (QRDR) and plasmid-mediated quinolone resistance genes. The susceptibility rates of these 17 Salmonella enteritidis isolates were 100% to ciprofloxacin and cefoxitin, these Salmonella isolates were 94.1%, these nalidixic acid and ceftriaxone isolates were 17.6%, and the gene detection rate of qnrB was 64.7%, 17 strains of Salmonella enteritidis GyrA gene mutation, the main mutation type Asp87Tyr; The drug resistance rates of 11 Salmonella havana isolates totrimethoprim, chloromycetin, sulfadimethisoxazol, sulfamethoxazole/trimethoprim, nalidixic acid, cefoxitin were 100%, 63.6%, 36.4%, 18.2%, 9.1%, 9.1% and the sensitive rate of ampicillin, amoxicillin/clavulanic acid was 100%, the detection rate of qnrB, qnrS is 9.1%, gene mutation type of 11 strains of Salmonella Havana ParC is Thr57Ser. Salmonella enteritidis and Salmonella havana drug resistance is serious in Xinjiang Urumqi. There should be concerns about the serious case of resistance to antimicrobial in Salmonella.In addition, the quinolone resistancedetermining region (QRDR) mutations and the plasmid-mediated quinolone resistance genes may affect the antimicrobial resistance of Salmonella enteritidis and Salmonella havana.
