GC-MS Analysis of Components Extracted from Oat Oil by Two Extraction Methods
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Abstract:
To study the effect of different extraction methods on the chemical composition of oat oil, the components of oat oil were extracted by conventional methods and by using supercritical carbon dioxide, and the composition of the resulting oat oils was determined and analyzed by gas chromatography-mass spectrometry (GC-MS). GC-MS analysis results showed that after the data were analyzed and searched against the National Institute of Standards and Technology (NIST) library, 6 and 13 components were identified in the oat oil extract obtained by traditional extraction and the methyl esterification product of fatty acids in the oat oil extract obtained by traditional extraction, respectively. There were 16 and 10 components identified from the oat oil extract obtained using supercritical carbon dioxide and the methyl esterification product of fatty acids in the oat oil extract obtained using supercritical carbon dioxide, respectively. The peak area normalization method was used to calculate the relative contents of the compounds; the chemical content of four samples accounted for 82.7%, 99.65%, 64.26%, and 99.71% of the total area, respectively. Among them, the compounds with a relatively high content in the oat oil were N-(2-hydroxyethyl)-decanamide, (Z)-6-octadecenoic acid, linoleic acid, oleic acid, hexadecanoic acid, and others. The study aims to provide a reference for further study and development of oat oil for edible or medicinal uses.
