Preparation and Identification of Monoclonal Antibody against Isoprocarb
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Abstract:
Isoprocarb is a common pesticide widely used in fruit, vegetable, and tobaccoin China. However, this pesticide also has strong biological toxicity. The aim of this study was to prepare a monoclonal antibody against isoprocarb, to facilitate rapid detection of this pesticide. In this study, 2-isopropylphenol and other compounds were used as raw materials to synthesize the hapten and artificial antigen of isoprocarb. Then, through immunized mice, cell fusion, cloning and screening, the monoclonal antibody cell was obtained, and monoclonal antibodies were obtained by ascites derivation and caprylic acid-ammonium sulfate precipitation. An indirect competitive enzyme-linked immunosorbent assay (ELISA) method was used to identify antibody specificity. The results showed that the monoclonal antibody detection range was from 1.88~82.8 ng/mL, with a half maximal inhibitory concentration (IC50) value of 11.7 ng/mL and an isoprocarb detection limit of 0.54 ng/mL. The antibody had high specificity, and no obvious cross-reactions with the isoprocarb structural analogs carbofuran, carbaryl, aldicarb, methomyl, or 2-isopropyl phenol. This antibody could be used to develop an ELISA assay for rapid isoprocarb detection. The preparation of this monoclonal antibody lays a solid foundation for the development of products to rapidly detect isoprocarb.
