Rapid Detection of Alopex lagopus- and Nyctereutes procyonoides-derived Constituents of Meat Products Using RT-PCR
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Abstract:
Alopex lagopus- and Nyctereutes procyonoides-specific primers and TaqMan probes were designed based on the conserved sequences in the mitochondrial genome of the Alopex lagopus and the displacement-loop (D-loop) region of Nyctereutes procyonoides. A real-time polymerase chain reaction (RT-PCR)-based method for the determination of Alopex lagopus- and Nyctereutes procyonoides-derived constituents of commercial meat products was developed in this study. The established RT-PCR method was evaluated for specificity, sensitivity, and linearity; the method showed good specificity and sensitivity, and the limits of detection for Alopex lagopus DNA and Nyctereutes procyonoides DNA were 0.5 pg/μL and 5 pg/μL, respectively. In summary, this RT-PCR method can be used for the identification of Alopex lagopus- and Nyctereutes procyonoides-derived constituents in commercial samples.
