Effect of Culture Conditions on the Growth and Hydrolytic Performance of Aspergillus niger
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Abstract:
The effects of different culture conditions, including different types and concentrations of nitrogen sources and surfactants, fermentation time, temperature, and rotation speed, on the biomass and hydrolytic activity of Aspergillus niger (A. niger) cells were studied in this paper. The results showed that different nitrogen sources enhanced the production of the induced enzymes with hydrolytic activities in A. niger cells, and the rate of enhancement by the different nitrogen sources were different. Among the tested nitrogen sources, yeast extract showed the best inducing effect on the cells. For yeast extract concentration of 1.5 g/L, the biomass of A. niger corresponds 1.35 g/L. Among the surfactants examined, the optimal hydrolytic activity was found in the A. niger cells cultured using Triton X-100. In the culture media with 0.5% Triton X-100, the hydrolysis efficiency of A. niger reached 58.12%. In addition, the effects of inorganic salts, culture temperature, and rotation speed were also investigated and the optimal conditions to cultivate Aspergillus niger cells with hydrolytic activities were as follows: yeast extract 1.5 g/L, Triton X-100 0.5%, magnesium sulfate heptahydrate 1 g/L, monopotassium phosphate 1 g/L, potassium chloride 0.5 g/L, ammonium sulfate 1.5 g/L, and anhydrous calcium chloride 0.1 g/L. The ideal culture temperature and rotation speed were confirmed as 35℃ and 140 r/min, respectively. Under the optimal culture conditions, biomass and hydrolysis efficiency of A. niger cells were 1.73 g/L and 90.23%, respectively.
