Isolation, Purification, and Characterization of Anti-inflammatory Activity of Truffle Polysaccharides
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Abstract:
Using Yunnan truffle as the raw material, black truffle polysaccharides (TPs) were obtained by water extraction and alcohol precipitation. The extracted black TPs were separated by DEAE Sepharose Fast Flow ion exchange chromatography to obtain two truffle polysaccharide fractions: TP1 and TP2. The inhibitory effects of TP1 and TP2 on the proliferation of mouse macrophages (RAW264.7) and the capacities of TP1 and TP2 for the phagocytosis of neutral red pigment were investigated. TP1 was further purified by Sephadex chromatography column to yield TP1-1, which was used for structural characterization. The results showed that TP1 and TP2 had no toxic effects on RAW264.7 cells; they could significantly increase the phagocytic capacity of RAW264.7 cells, and the effect of TP1 was greater than that of TP2. The average molecular weight of TP1 was 757910 u, and its monosaccharide composition was D-glucose and D-mannose, in proportions of 88.94% and 1.19%, respectively. Ultraviolet (UV) spectra revealed that TP1-1 did not contain protein or nucleic acid. The results of infrared spectrum analysis showed that TP1-1 had a pyran ring structure and contained alpha-glycosidic bonds and mannose.
