Protective Effect of Water Extracts of Orychophragmus violaceus Seeds on Alcohol-induced Acute Liver Injury in Mice
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Abstract:
The mouse model was used to study the protective effect of water extracts of Orychophragmus violaceus seeds on alcohol-induced acute liver damage. Male ICR (Institute for Cancer Research) mice were randomly divided into six groups: normal group, model group, low-dose water extract group (125 mg/kg), medium-dose water extract group (250 mg/kg), high-dose water extract group (500 mg/kg), and bifendate (150 mg/kg) positive control group. All groups were administered the respective extracts or drug continuously for four days, except for the normal and model groups. Then, alcohol-induced acute liver injury was established in the model and experimental groups by giving each mouse 12 mL/kg 56° Red Star Erguotou at 1 h after the last extract/drug administration. The animals in the normal group were given an equal volume of distilled water instead. The mice were sacrificed after 16 h, and the activities of several key enzymes (the alanine transaminase (ALT), aspartate transaminase (AST), triglyceride (TG) and those in the liver tissue (superoxide dismutase (SOD), alcohol dehydrogenase (ADH), glutathione peroxidase (GSH-Px), and glutathione (GSH) ), were determined. The liver organ index was calculated, and hematoxylin and eosin staining was used to observe the pathological changes in the liver tissue. The seed water extracts could significantly reduce the serum ALT and AST activities and TG level (p<0.05 or p<0.01), and suppress liver steatosis in the mice with alcoholic liver injury, relative to the results in the model mice. Meanwhile, the activities of SOD, ADH, GSH-Px, and GSH in the liver tissue homogenate supernatant were significantly increased in the low- and medium-dose groups (p<0.05, p<0.01). In conclusion, the water extract of Orychophragmus violaceus seeds had a significant protective effect on acute alcoholic liver injury in mice.
