Tetracycline Detection in Dairy Products by Using Hemin with Functional Nucleic Acid-enhanced Catalytic Activity
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Abstract:
In order to establish a convenient and effective method for the colorimetric detection of tetracycline (TET) residues, guanine-rich single stranded deoxyribonucleic acid (G-ssDNA) was first used to enhance the horseradish peroxidase activity of hemin, which oxidized 3,3?,5,5?-tetramethylbenzidine (TMB) to form a yellow product. With the addition of TET in the system, the catalytic activity of hemin was suppressed, and the substrate was oxidized to form a blue product. The TET content could be determined by measuring the change in the absorption spectra of the reaction products. Under optimal measurement conditions, the proposed method has a detection limit of 5.69 nM, and a linear range from 10 nM to 100 nM, with a correlation coefficient of 0.994. The specificity test confirmed that other related antibiotics did not show significant cross-reactivity with the TET measurement. This method was used to detect TET in milk samples, and the spiked recoveries for TET detection were in the range of 92% to 99%. The advantages of the method established in this work include high specificity and convenient operation. In addition, the method allows for rapid TET detection without relying on large instruments, and thus can be widely used for the detection of TET residues in dairy products.