UPLC-Triple-TOF-MS/MS Analysis of the Phospholipid Composition of Human Milk from the Northeast Regions of China
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Abstract:
Ultra-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight tandem mass spectrometry (UPLC-Triple-TOF-MS/MS) was used for the qualitative and relative quantitative analysis of phospholipids in human milk during different lactation periods, and to provide a reference for the analysis of phospholipid composition of milk from Chinese women. A total of 73 human milk samples including colostrum (1~5 d), transitional milk (6~20 d), and mature milk (after 21 d) were collected from Harbin (Heilongjiang province), Qiqihar (Heilongjiang province), and Jilin (Jilin province). LipidView 1.2, PeakView 2.2 with MasterView? 1.1 plugin, and MultiQuant? were used for the molecular formula search, qualitative analysis, and relative quantitative analysis of phospholipids, respectively. Sixty species of phospholipids were identified in the milk samples, and the numbers of phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), phosphatidylserines (PSs), phosphatidylinositols (PIs), and sphingomyelins (SMs) were 17, 25, 4, 5, and 9, respectively. The relative contents of PCs, PEs, SMs, PSs, and PIs in the total phospholipids were 38.12%, 26.97%, 29.54%, 4.43%, and 0.94%, respectively. In addition, the relative contents of the various phospholipids varied with the lactation period, although the changes in the relative contents of most phospholipids were not significant.
