Determination of Selenium in Rat Tissues after Oral Administration of Selenium-enriched Green Tea by Graphite Furnace Atomic Absorption Spectrometry
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Abstract:
A method for the determination of selenium (Se) concentrations in rat plasma, heart, liver, testicle, pancreas, lung, nail, brain, kidney, and muscle by graphite furnace atomic absorption spectrometry (GFAAS) was developed and validated. The content and distribution of selenium in rat tissues after oral administration of selenium-enriched green tea were then determined. In this assay, male rats were randomly divided into selenium-enriched group, non-selenium-enriched group, and blank group (n=6), which were given 10 g/kg selenium-enriched green tea, non-selenium-enriched green tea, and 10 mL/kg ultra-pure water once per day for 30 d, respectively. The rat tissues were wet-digested and the selenium contents were determined by GFAAS. The standard curve was constructed using the standard addition method, and the result of method validation showed that the developed GFAAS method exhibited a good linearity over the concentration range of 10~100 ng/mL (r≥0.99136). The intra-batch and inter-batch precision were all within 12.2% at three concentration levels. The mean accuracies and recoveries were 89.5%~106.9% and 86.1%~95.1%, respectively. The results of selenium distribution in tissues showed that the selenium contents in rat heart, testicle, lung, liver, kidney, and pancreas in the selenium-enriched green tea-treated group increased significantly compared to those of the blank group, and the selenium level in the pancreas increased significantly compared with that of the non-selenium-enriched green tea-treated group. In conclusion, the developed GFAAS method of optimizing sample preparation and constructing the standard curve for each tissue is accurate and reproducible, and is suitable for the determination of selenium content in rat tissues after administration of selenium-enriched green tea. The selenium in the enriched green tea could be absorbed by rats, and the selenium contents in all rat tissues were lower than the toxic level.
