Protective Effects of Dendrobium candidum on Ischemia Reperfusion- Induced Arrhythmia
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Abstract:
The protective effects and underlying mechanisms of Dendrobium candidum on ischemia reperfusion-induced arrhythmia were explored. Healthy male Sprague Dawley (SD) rats were randomly divided into five groups (n=12), including control group, model group, and three Dendrobium candidum groups, high-dose [1080 mg/(kg?d)], medium-dose [180 mg/(kg·d)], and low-dose [45 mg/(kg?d)]. After intragastric administration for 30 d, except for the control group, ligation of the anterior interventricular branch of the left descending coronary artery was performed to establish a mouse model of myocardial ischemia reperfusion-induced arrhythmia. Lead II electrocardiograms were recorded, and the time of arrhythmia onset, duration of arrhythmia, and incidences of ventricular premature (VP) beats, ventricular tachycardia (VT), and mortality in each group were examined and recorded at different reperfusion time points (15 min, 30 min, 1 h, 2 h, 4 h, and 24 h). Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and cardiac troponin I (cTn-I) in plasma. Superoxide dismutase (SOD) and malondialdehyde (MDA) levels in myocardial tissue were determined using corresponding test kits. The results showed that Dendrobium candidum could shorten the recovery time after arrhythmia and significantly decrease the arrhythmia score. The ANP, BNP, and cTn-I levels in plasma of the rats with ischemia reperfusion-induced arrhythmia were increased, the SOD level in myocardium was decreased, and the MDA level was increased. Dendrobium candidum could inhibit these effects. This study indicated that Dendrobium candidum showed a protective effect on ischemia reperfusion-induced arrhythmia, and the mechanism might involve its antioxidant activity.
