Preparation of Monoclonal Antibody and Colloidal Gold Immunochromatographic Test Strip against Furazolidone Metabolites
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Abstract:
The aim of the study was to establish a method for the rapid detection of the furazolidone metabolites. A conjugate of the derivatives of furazolidone metabolites and bovine serum albumin (BSA) was used to immunize mice. The cells were prepared by monoclonal antibody technology, and the positive clones were screened by indirect ELISA and icELISA. Monoclonal antibodies purified from ascitic fluid were used in the colloidal gold tag and for the preparation of the colloidal gold immunochromatographic test strip. Two cell types were obtained, which could stably secrete antibodies. The titer of the purified antibody named 4G3 was 1:106, and the median inhibitory concentration (IC50) was 1.7 μg/L with affinity constant Ka=1.6×109 L/mol. The results showed that the detection using the test strips was limited to four μg/L, there were no cross reactions between CPAHD, CPSEM, and CPAMOZ, and the test results of the samples were consistent with the results of HPLC. In this study, specific monoclonal antibodies against CPAOZ were prepared, and colloidal gold immunochromatographic test strips were developed based on the antibodies, which could achieve rapid and sensitive detection of furazolidone metabolite residues.
