Optimal Conditions for Rapid Detection of Salmonella spp. via Potassium Manganese Trifluoride Nanoprobe-based Nuclear Magnetic Resonance
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Abstract:
This work attempted to establish a new method for the rapid detection of Salmonella spp., using a nuclear magnetic resonance (NMR) method based on potassium manganese trifluoride (KMnF3) nanoprobes. Immunomagnetic beads were produced by coupling an anti-Salmonella monoclonal antibody with carboxyl magnetic beads, which were used as NMR molecular probes and biological sensors to specifically capture and detect the pathogenic bacteria in samples, in order to create a more rapid method to detect Salmonella spp. The results showed that the relaxation time in sample detection was influenced to various degrees by the amount of added activator, the choice of buffer solution for the capture of Salmonella spp., the bacterial incubation time, and the volume of added NMR probes. The capturing conditions were optimized as follows: the mass ratio of potassium manganese trifluoride to 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride- and N-hydroxysulfosuccinimide-modified magnetic beads at activation was 1:5; 80 μL of antibody-potassium manganese trifluoride was required to specifically capture Salmonella spp. incubated for 10 h; sterile distilled water was used as the buffer. A new method and approach for the detection of Salmonella spp. was provided in this study, and the method shortened the detection time and expanded the application of low-field nuclear magnetic resonance in the field of food safety.
