Adsorption and Separation of Monascus Yellow Pigments in Monascus Fermentation Broth
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Abstract:
The adsorption and isolation capacities of macroporous resins with different polarities, ion exchange resins, activated carbon, and silica gel on water-soluble yellow pigments of submerged Monascus fermentation broths were studied. The results showed that nonpolar macroporous resin DA201-C exhibited the best performance; the adsorption isotherm of DA201-C was well fitted with the Freundlich equation, and the adsorption kinetics followed the pseudo-first-order kinetics model, indicating that adsorption was not restricted to monolayer adsorption. Liquid film diffusion was the major rate-limiting step, and the diffusion rate constant was 0.16 min-1. The purification conditions were optimized by static and dynamic adsorption and desorption experiments, and the results were analyzed using SPSS software. The optimal desorption performance was achieved when the solid material-to-liquid ratio of static adsorption and desorption was 1:2, both the adsorption and desorption times were 15 min, the eluent was 100% ethanol, and the eluent was acidic. When the flow rate of dynamic adsorption and desorption was 0.25 BV/min, the water-soluble Monascus yellow pigments could be obtained with high recovery (96.99%), desalination (99.44%), and desugarization rate (92.52%). The preliminary scale-up experiment showed a stable adsorption and desorption efficiency. In conclusion, the method using DA201-C resin to isolate and purify the water-soluble Monascus yellow pigments in fermentation liquid is easy to operate, has a short cycle and good performance in a scale-up experiment, and shows a great application value in industrial production.
