Abstract:Rose oil distillation wastewater (RODW) is the waste solution that obtained after the preparation of rose essential oil by water vapor extraction of roses. The phytochemical constituents of RODW and their biological activities were investigated in this study. Gas chromatography-mass spectrometry (GC-MS) quantitative analysis combined with high-performance liquid chromatography (HPLC) showed that the main chemical components of RODW included linalool, phenethyl alcohol, citronellol, α-bisabolol, and kaempferol-3-O-rutinoside, and the antioxidant, antimicrobial, and tyrosinase inhibitory activities of RODW were systematically evaluated. The results showed that the half maximal inhibitory concentration (IC50) values for tyrosinase inhibitory activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity of RODW were 2.45±0.14 mL, 2.58±0.04 mL, and 3.425±0.21 mL, respectively. Furthermore, RODW possessed strong antimicrobial activity, and showed a high inhibition rate against Fusobacterium nucleatum, with a minimum inhibitory concentration (MIC) of 32 μL and minimum bactericidal concentration (MBC) of 125 μL. This study provides a theoretical basis for the application of RODW in cosmetics and food industries.
