Abstract:To study the synergistic effect of an acidic Auricularia auricular polysaccharide (Ac-AAP) with other polysaccharides on anticoagulant activity, tea polysaccharide (TP), jujube polysaccharide (JP), and seaweed polysaccharide (SP) were combined with Ac-AAP, respectively. The Chou-Talalay combination index (CI), percentage inhibition of conversion of fibrinogen (FIB) to fibrin, activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) were used as indicators to evaluate the synergistic effect of Ac-AAP with other three polysaccharides on anticoagulant activity. The experimental results revealed that the Ac-AAP:SP (1+2) combination had the strongest anticoagulant activity (p<0.05), and the CI value was 0.63175. Comprehensive evaluation of the anticoagulant activity showed that with a polysaccharide concentration of 2.0 mg/mL, values of all anticoagulation indices of Ac-AAP:SP (1+2) were better than those of the other combinations (p<0.05); the percentage inhibition of conversion of FIB to fibrin was 91.06±0.79 %, the APTT of plasma was 95.47±0.61 s, and the TT value was 62.24±0.53 s, but the PT of plasma was not significantly different from that of saline (negative control).
