Effects of Physalis alkekengi Extracts with Different Polarities on In Vitro Induced Nonalcoholic Fatty Liver Cells
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Abstract:
The aim of this study was to investigate the effects of Physalis alkekengi extracts with different polarities in an in vitro model of induced nonalcoholic fatty liver disease (NAFLD) and identify the possible mechanism. Physalis alkekengi samples were extracted using organic solvents with different polarities. The concentrations of medical fat emulsion for injection and Physalis alkekengi extracts were selected by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Lipid droplets stained with oil red O were observed under a light microscope. The triglyceride (TG), alanine aminotransferase (ALT), aspartate transaminase (AST), and gamma-glutamyl transpeptidase (GGT) levels were determined by an automatic biochemical analyzer. Compared with normal liver cells, HL7702, the model group showed a large quantity of orange-red lipid droplets in the cytoplasm after oil red O staining, and the intracellular TG, ALT, AST, and GGT levels increased significantly (p<0.01). Compared with the model group, the groups treated with different Physalis alkekengi extracts for 12 h exhibited a considerably reduced number of orange-red lipid droplets in the cytoplasm and significantly decreased levels of intracellular TG, ALT, AST, and GGT (p<0.01). An in vitro cell model of nonalcoholic fatty liver disease was successfully established, and treatment with Physalis alkekengi extracts with different polarities could reduce the accumulation of TG and leakage of ALT, AST, and GGT in NAFLD cells.
