Study on Biomarkers of Foodborne Pathogenic Salmonella Serotypes
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Abstract:
Identifying the characteristic metabolites of foodborne pathogenic Salmonella serotypes is the basis for the research and development of an identification and monitoring technique for foodborne pathogenic microorganisms. In this study, intracellular metabolites were extracted from five different Salmonella serotypes comprising eight standard strains by using metabolomics technology, and intracellular metabolic profile analysis was conducted for each strain. Additionally, fingerprints were constructed and principal component analysis (PCA) was performed. It was found that the metabolites of each serotype had large differences in terms of type and abundance, and some potential characteristic metabolites were found. For example, mercaptoacetic acid and α-hydroxypentanedioic acid were biomarkers of the S. enteritidis serotype; N-acetyl aspartic acid, 2(1H)-pyrimidone, and L-threonic acid were only produced by the S. typhi serotype; tetradecanoic acid methyl ester and glyceryl ether-glucoside were released only by S. typhimurium serotype; 1,4-naphthoquinone was only produced by S. choleraesuis serotype, and 3-pyridinecarboxylic acid trimethylsilyl ester was unique to S. arizonae serotype. The five Salmonella serotypes could be clearly distinguished by cluster analysis and PCA. As a result, the above specific products could be used as potential biomarkers of Salmonella serotypes.
