Study of a Gel-based Immunoassay Method for the Rapid Visual Detection of Furaltadone Metabolites
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Abstract:
A new method using an immuno-affinity column for the detection of metabolites of the veterinary drug furaltadone (AMOZ) was established in this study, and was applied for the rapid visual detection of AMOZ residues in animal-derived foods. Measurement was conducted in a standard solid phase extraction cartridge (1 mL) that consisted of a detection layer, containing anti-furaltadone metabolite antibody-coupled gel, and a quality control layer with anti-HRP antibody-coupled gel. The sample extract was pre-mixed with the enzyme-labeled antigen and the mixture was added to the detection column. Based on the direct competitive immuno-reaction and the HRP enzymatic reaction, qualitative analysis was performed according to the presence and strength of color, and the content of AMOZ was determined semi-quantitatively. The whole procedure was completed within 10 min, and the limit of detection of the gel-based detection column for the detection of furaltadone metabolite derivatives (NPAMOZ) was 20 μg/L. The visual detection limits of this method for the detection of AMOZ in animal-derived foods (beef, snapper, chicken, shrimp, pork, squid, chicken liver, and yellow croaker) were 3 μg/kg. This gel-based visual immunoassay method has high accuracy and good specificity and is easy to use; it is suitable for the rapid detection of AMOZ residues in a large number of animal-derived food samples.
