Quantitative Detection of Six Classes of Antibiotic Resistance and Class Ⅰ Integron Genes in Aquatic Products
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Forty-eight aquatic products from two farmers’ markets, one seafood market, and three supermarkets in the Tianhe district of Guangzhou were used in this study, and the total DNA from three aquatic products-shrimps, fish, and shellfish-was extracted. Quantitative real-time polymerase chain reaction (PCR) was used to quantify six classes of antibiotic resistance genes (ARGs; tetA, tetB, tetM, sulII, floR, aphA-1, aadA, ermB, and cmlA), the intl1 gene on the 5′ end of class 1 integron, and the sulfanilamide resistance gene sul1 and quaternary ammonium salt compound and ethidium bromide resistance gene qaceΔ 1 on the 3′ end of class Ⅰ integron. The results revealed that all six classes of ARGs were detected in all aquatic products, and the abundance varied over seven orders of magnitude (102~108); the minimum and the maximum values were 1.86×102 copies/g and 8.98×108 copies/g, respectively. Among the three kinds of aquatic products, ARGs were present at the highest concentration in shrimps and the lowest concentration in shellfish. Among the three sampling sites, farmers’ markets had the highest concentration of ARGs, followed by the seafood market and the supermarkets. This study lays the foundation for further research on foodborne diseases caused by antibiotic resistant bacteria with ARGs and mobile integron resistance components.
