Effects of Inorganic Nitrogen Sources on the Molecular Weight of Pullulan Produced by Aureobasidium pullulans
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Abstract:
The effects of five types of inorganic nitrogen sources on the yield, structure, purity, and molecular weight of pullulan were studied using Aureobasidium pullulans CGMCC No. 11062 as the parental strain. The results showed that the yield and molecular weight of pullulan were significantly influenced by the types of inorganic nitrogen source, whereas the structure and purity of pullulan were not affected. When ammonium sulfate (1.5 g/L) was used as the sole nitrogen source, the yield of pullulan was 32.84 g/L, and the maximum weight-average molecular weight (Mw) of pullulan (799823 ku) was obtained. Furthermore, the yield and molecular weight of pullulan were significantly influenced by the concentration of ammonium sulfate, which did not significantly affect the structure of pullulan. With increasing concentration of ammonium sulfate, the yield and molecular weight of pullulan increased simultaneously. The maximum yield of pullulan (32.45 g/L) was reached at 1.5 g/L ammonium sulfate, and the peak Mw of pullulan (1236058 ku) was obtained at 2.1 g/L ammonium sulfate. The purity of pullulan produced from all experiments was between 95% and 99%. These studies could provide technical guidance for the production of pullulan with different molecular weights.
