To explore the effect of 6-benzylaminopurine (6-BA) treatment on the respiratory pathway and energy level in postharvest broccoli florets, samples were treated with water (as the control) and with 30 mg/L 6-BA, respectively, and then stored at (15±1) ℃ and 90%~95% relative humidity. The effect of 6-BA treatment on the exterior quality, total respiration intensity, the change of respiratory pathways, including Krebs cycle (TCA) pathway, pentose phosphate pathway (PPP), cytochrome pathway (CP), and alternate pathway (AP), the accumulation of superoxide anion, as well as the levels of adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP), and the energy status in postharvest broccoli florets were investigated. The results showed that 6-BA treatment delayed the yellowing of broccoli, significantly decreased the total respiration intensity and the activities of CP and PPP, and increased the expression level of AP. In addition, in the late stage of storage (six to ten days), 6-BA treatment reduced the proportion of TCA activity, promoted the accumulation of ATP, and delayed the decrease in the level of energy charge in postharvest broccoli, inhibiting the accumulation of superoxide anion in postharvest broccoli florets. Thus, the inhibitory effect of 6-BA on the senescence of postharvest broccoli florets might be associated with the regulation of respiratory pathways and the maintenance of energy balance in the broccoli tissues.
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