Influence of Different Medium Conditions on Molecular Typing of Listeria monocytogenes by Enterobacterial Repetitive Intergenic Consensus PCR
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Abstract:
Twenty-six strains of Listeria spp. stored in the laboratory were used as the experimental object here, and the enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) and gel electrophoresis techniques were applied to genotyping. Furthermore, according to the typing results, strains of different subtypes were selected, and the effects of different medium conditions on the results of ERIC-PCR molecular typing of Listeria spp. were explored by changing the culture conditions. The preliminary test results showed that 26 strains of Listeria spp. in the laboratory could be subdivided into at least 11 genotypes, and the number of the strains belonging to genotype I was the highest, whereas the numbers of strains belonging to genotypes III, VII, VIII, IX, X, and XI were the lowest. In addition, the results revealed that the changes in medium and culture conditions affected the typing results of LM89 and LM109 (wild-type Listeria monocytogenes), which belong to the genotypes with the lowest number of strains. From this preliminary study, it can be concluded that different nutritional conditions and culture conditions may have an impact on the genomic stability or gene expression of Listeria spp. Therefore, the host source and culture conditions must be taken into account during basic research on L. monocytogenes pathogenicity and its molecular typing.
