Effect of Self-developed Milk Powder on Oxidized-low Density Lipoprotein (ox-LDL)-Induced Proliferation of Vascular Smooth Muscle Cells
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Abstract:
The effect of self-developed milk powder on the proliferation and nitric oxide (NO) production of vascular smooth muscle cells (VSMC) induced by oxidized-low density lipoprotein (ox-LDL) was studied. A VSMC proliferation model was established by inducing the proliferation of VSMC with ox-LDL. The effects of the milk powder developed in our laboratory was evaluated by comparing its inhibitory effect on the proliferation and NO production of the model cells with three kinds of common commercial milk powders. The results indicated that when VSMCs were treated with 30 μg/mL ox-LDL for 24 h, cell proliferation was significantly promoted and NO release was inhibited (p<0.01), indicating that the model was successfully established. In this VSMC model, different amounts of self-developed milk powder were added, and the results showed that solutions of 200, 400, and 800 μg/mL milk powder inhibited ox-LDL-induced VSMC proliferation. In addition, 400 μg/mL milk powder highly inhibited cell proliferation and promoted NO production (p<0.01). The above findings demonstrate that self-developed milk powder inhibited the ox-LDL-induced proliferation in VSMCs; therefore these milk powders may prevent atherosclerosis and be beneficial for middle-aged and older populations.
