Screening and Anti-inflammatory Activities of Natural Plant Extracts with a Protective Effect against High-glucose-induced Injury to HBZY-1 Cells
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Abstract:
Active ingredients (from the extracts of five functional food plants and Salvia) with an anti-inflammatory effect on high-glucose-induced injury to HBZY-1 cells were screened here. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure cell proliferation in the initial screening, and lactate dehydrogenase (LDH), total nitric oxide synthase (TNOS), and inducible nitric oxide synthase (iNOS) were quantified by the microplate method. The concentrations of nitric oxide (NO) and endothelin (ET-1) were determined by the nitrate reductase method and enzyme-linked immunosorbent assay (ELISA), respectively. Hematoxylin and eosin (HE) staining was used for examining morphological changes in these cells. The results showed that the extracts of polysaccharides from Lycium barbarum (LBP), flavonoids from mulberry leaves, and flavonoids from Ginkgo biloba leaves had a protective effect against the high-glucose-induced proliferation of HBZY-1 cells in a concentration-dependent manner. The optimal concentrations of the three extracts were 400, 350, and 125 μg/mL, respectively. The three above-mentioned extracts inhibited the secretion of LDH, ET-1, and iNOS and promoted the production of NO. In addition, the amount of LDH yielded by the extract of Ginkgo biloba leaves was 717.86 U/(g prot), which was close to that of the positive control, and the production of NO was 16.14 μmol/L, which was higher than that of the positive control. The amount of ET-1 yielded by the extract of mulberry leaves was 26.42 pg/mL, which was higher than that of the positive control. All the three extracts protected and maintained cell morphology well. All the three active ingredients in this experiment were found to have an anti-inflammatory effect on the HBZY-1 cell injury induced by a high concentration of glucose and can be used as functional food ingredients for improving the health of patients with renal disease.
