Immunological and Antitumor Activities of Polypeptides from Pleurotus eryngii Mycelium
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Abstract:
The aim of the present work was to study the immunological and antitumor activities of polypeptides from Pleurotus eryngii mycelium, which was used as the raw material. The immunological activities of the polypeptides were investigated by measuring neutral red uptake capacity, the ability to stimulate macrophages to secret nitric oxide and hydrogen peroxide, the levels of the secreted cytokines tumor necrosis factor-α and interleukin-6, the expression levels of the membrane surface proteins toll-like receptor (TLR)2 and TLR4, and Ana-1 cell survival rate. The antitumor activities of the polypeptides were studied through the inhibition of the proliferation of human breast cancer cells (BT-549), cervical cancer cells (Hela-229), and human gastric cancer cells (HGC-27). The results showed that within a concentration range of 0.05~2 mg/mL, the Pleurotus eryngii mycelium polypeptide could, to some extent, promote the proliferation of macrophages, enhance TLR2 and TLR4 expression levels and macrophage neutral red uptake, and increase the secretion of nitric oxide and hydrogen peroxide by macrophages. The polypeptide had strong inhibitory effects on the proliferation of the breast cancer, cervical cancer, and human gastric cancer cells. These results indicate that the polypeptides from Pleurotus eryngii mycelium had good immunological and antitumor activities with a concentration-response relationship.
