Effects of Different Culture Conditions on Callus Growth and Chlorogenic Acid Accumulation in Gardenia jasminoides Ellis
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Abstract:
Using the growth index, the content of chlorogenic acids, and the production of chlorogenic acid per liter of medium as evaluation criteria, the effects of different culture media, carbon sources, sucrose concentrations, illumination conditions, and casein hydrolysate (CH) concentrations on callus growth and the accumulation of the secondary metabolite chlorogenic acids in Gardenia jasminoides Ellis were studied. The results indicated that different culture conditions had significant effects on the growth of Gardenia jasminoides Ellis and the accumulation of chlorogenic acids, and conditions suitable for growth were not optimal for the accumulation of chlorogenic acids. The optimal conditions for growth were as follows: Murashige and Skoog (MS) medium was used as basal medium, sucrose and maltose were used as carbon sources (at a concentration of 3%), and an alternating period of light and dark was applied. Under the above conditions, the highest fresh weight gain index was obtained (47.11%). The optimal conditions for the accumulation of chlorogenic acids were as follows: MS medium, 5% sucrose as the carbon source, no added CH, and application of an alternating period of light and dark. Under the above conditions, the highest yield of chlorogenic acids was 35.55 mg per liter of medium.
