Antibiotic Resistance and Random Amplified Polymorphic DNA PCR Typing of Pseudomonas aeruginosa Isolated from Barreled Water
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Abstract:
The Kirby-Bauer disk diffusion method and random amplified polymorphic DNA (RAPD) typing were used to study the antibiotic resistance and the genetic diversity of 23 waterborne Pseudomonas aeruginosa strains. The susceptibility test showed that the drug resistance rates of the 23 P. aeruginosa isolates to sulfamethoxazole/trimethoprim (SXT), tetracycline (TE), and minocycline hydrochloride (MH) were 69.4%, 13.2%, and 39.2%, respectively, while they showed an almost 100% susceptibility to the other 13 antibiotics tested. Cluster analysis of the RAPD-polymerase chain reaction (PCR) fingerprints showed that at a similarity coefficient of 62%, the strains were grouped into five clusters (A~E, cluster C as the dominant cluster) and four clusters (F~I, clusters I and G as the dominant clusters) by primers 208 and 272, respectively. Among 23 strains, those with no drug resistance were mostly grouped into cluster B, three multidrug-resistant isolates were grouped into cluster D, and cluster I contained most of the isolates that were resistant to SXT and MH. The results from this study revealed that waterborne P. aeruginosa strains have a high genetic diversity and multiple drug resistance, and provide corresponding supporting data for the tracking and control of waterborne P. aeruginosa contamination.
