Effect of 1-Deoxynojirimycin on Lipid Metabolism in Normal Mice and Preliminary Exploration of the Underlying Mechanism
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Abstract:
To explore the effect of 1-deoxynojirimycin (DNJ) on the lipid metabolism, and the corresponding mechanism, normal mice were fed DNJ by gavage for 40 days, and then biochemical indicators of lipid metabolism, the activity of enzymes, and the antioxidant indices of the animals were determined using enzyme-linked immunosorbent assay (ELISA) kits. Compared with the negative control group, 8.0 mg/kg.bw/d dosage of DNJ decreased the rates of weight gain by 59.40% (female) and 9.15% (male), decreased intraperitoneal fat coefficients by 38.30% (female) and 15.18% (male), and decreased the contents of liver fat by 18.42% (female) and 36.66% (male)The addition of DNJ also decreased the contents of serum total cholesterol (TC) by 27.40% (female) and 28.57% (male), and decreased the contents of triglycerides (TG) in serum by 0.89% (female) and 15.03% (male). Besides, this dose increased the contents of high-density lipoproteins (HDL) in serum by 42.19% (female) and 6.80% (male), increased the activities of superoxide dismutase (SOD) in liver by 29.24% (female) and 25.17% (male) increased the contents of glutathione (GSH) in liver by 16.29% (female) and 15.00% (male), and decreased the contents of 8-isoprostane in liver by 18.53% (female) and 15.19% (male). It was also found that 8.0 mg/(kg bw?d) dosage of DNJ increased the activities of hepatic fatty acid synthase (FAS) and acyl-CoA oxidase (ACO) in female mice by 14.02% and 9.67%, respectively, and increased the activity of ACO in the liver of male mice by 21.63%. The results indicated that an appropriate dosage of DNJ could reduce fat accumulation by inhibiting fatty acid synthesis in female mice and accelerating fatty acid oxidation in male mice; a better effect on obesity prevention was shown in female mice. DNJ could also enhance the body's antioxidant ability to prevent abnormal lipid metabolism.
