Rapid Detection of Salmonella Using Carbohydrate Microarrays
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Abstract:
The applicability of carbohydrate microarrays for Salmonella detection was investigated, using Salmonella ATCC31685 as the primary test subject. Different types of carbohydrate microarrays were first tested to optimize the detection conditions. Then, using the optimized conditions, decreased carbohydrate concentrations or bacterial concentrations were used to determine the microarray’s limit of detection. The carbohydrate microarrays were tested for their destructiveness of bacterial strains as well as their ability to screen for antagonists of Salmonella lectin FimH. The results indicated that Salmonella bound to modified mannose compounds in the carbohydrate microarrays, among which the microarray chips with Man-1 and NHS were the most optimal. In addition, the microarray-binding abilities of two Salmonella strains (ATCC31685 and ATCC9184) were found to be different. Therefore, carbohydrate microarrays can serve as a rapid procedure for detecting Salmonella. At a carbohydrate concentration of 313 mmol/L or a cell concentration of 106/mL, the fluorescent signal approaches a background value. The carbohydrate microarray detection of Salmonella is non-destructive and can be used continuously. In addition, these microarrays can be used as tools for screening FimH protein antagonists.
