Determination of Nine Phytotoxins in Botanical Beverage by Solid-phase Extraction and Gas Chromatography-mass Spectrometry
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Abstract:
A sensitive method was established for the simultaneous determination of nine phytotoxins, including thujone, estragole, pulegone, safrole, methyl eugenol, coumarin, santonin, and α-and β-asarone, in botanical beverage by solid-phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS). Samples were extracted and purified using hydrophilic lipophilic balanced (HLB) SPE cartridges after high-speed centrifugation. Finally, the eluent was concentrated by nitrogen gas blowing and all samples were separated using DB-5MS capillary chromatographic column and detected by GC-MS with selected ion monitoring (SIM). The results indicated good linear relationships in the range of 5~1000 μg/L for the nine phytotoxins, with correlation coefficient >0.999. The limits of detection (LODs, S/N=3) and quantification (LOQs, S/N=10) of the method for the target compounds were in the range of 0.3~1.4 and 1.0~4.7 μg/kg, respectively. The mean recoveries of negative samples at three spiked levels were between 76.3% and 99.7%, and the relative standard deviation (RSD, n=6) was <8.7%. This method was suitable for the identification and quantification of nine phytotoxins in botanical beverage owing to its simplicity and accuracy. Among eight actual samples tested, one sample of herb beverage was found containing 0.7 mg/kg methyl eugenol .
