Synthesis and Characterization of Luteolin Complexes and Their Inhibition Effects on Xanthine Oxidase
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Abstract:
The coordination effect was used to synthesize luteolin complexes between luteolin and metal ions Zn2+ and Mn2+, and the complexes were examined by ultraviolet UV-visible spectroscopy, infrared spectroscopy, thermogravimetric-differential scanning calorimetry, and elemental analysis techniques. Moreover, the inhibition of xanthine oxidase by the luteolin complex was studied, the inhibitory capacities of luteolin and its complexes were compared, and the type of inhibition of xanthine oxidase was determined. The results indicated that coordination reactions occurred between luteolin and the metal ions Zn2+ and Mn2+ to form stable complexes. The chelation site was located at the 5-OH of ring A and 4-C=O of ring C. Compared to luteolin, its complexes exhibited a better inhibitory effect on xanthine oxidase. When the xanthine substrate concentration was 0.6 mM, the graph of enzyme concentration versus enzyme activity showed that the types of inhibition by luteolin and its complexes were reversible. When the concentration of xanthine oxidase was 10 μg/mL, the double reciprocal Lineweaver-Burk plot showed that the types of inhibition by luteolin and its complexes were competitive.
