Abstract:The adhesive ability of eight strains of Lactobacillus plantarum on the HT-29 intestinal epithelial cell line, and the inhibitory effect of the strain with the highest adhesive ability and its exopolysaccharides (EPS) on Escherichia coli-induced IL-8 production and E. coli adhesion on HT-29 cells were investigated. The results indicated that the eight Lactobacillus strains adhered to HT-29 cells to different degrees, and L. plantarum 35 had the highest adhesive ability. L. plantarum 35 could protect HT-29 cells from E. coli adhesion by displacement, competition, and exclusion, with inhibition rates of 30%, 33%, and 59%, respectively. At an active concentration of 500 μg/mL, EPS could inhibit E. coli adhesion by 32%. L. plantarum 35 could inhibit E. coli-induced IL-8 production by HT-29 cells by displacement, competition, and exclusion with inhibition rates of 3%, 28%, and 40%, respectively. EPS could inhibit E. coli-induced IL-8 production by HT-29 cells in a dose dependent manner, with the highest inhibitory rate of 50% at 500 μg/mL concentration. L. plantarum 35 had no effect on IL-10 production by HT-29 cells. The results showed that L. plantarum 35 has a potential probiotic function and may inhibit inflammatory bowel disease induced by E. coli.
