Metabolomics Study of Pinus massoniana Pollen Extract and its Preventive Effect on Benign Prostate Hypertrophy in Mice
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Abstract:
The effect of masson pine (Pinus massoniana) pollen extract (MPPE) on benign prostate hypertrophy (BPH) in mice was studied. Male KM mice were randomly divided into six groups: normal control group, BPH model group, 20, 4080 mg/kg MPPE groups and 0.8 mg/(kg?d) finasteride group. After two weeks of advanced intragastric MPPE administration, an intraperitoneal injection of testosterone propionate was used to initiate the BPH, following which the MPPE or finasteride treatment was administered intragastically for 4 weeks. At the end of the treatment period, morphological changes in the tissues were observed, and levels of the cytokines IL-6, IL-1β, and TNF-α as well as of PACP, DHT, NO, and NOS were measured. Ultraperformance liquid chromatography-mass spectrometry was employed to detect changes in serum and urine metabolites. Partial least squares discriminant analysis was used for group differentiation and biomarker selection. All three doses of MPPE decreased the prostate index, inflammatory cytokines, DHT concentration, PACP and NOS activities, and NO production. Serum metabolomics analysis found and identified three potential biomarkers: 1-palmitoyl-sn-glycero-3-phosphocholine, 1-O-hexadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine, and cis-13-docosenoamide. MPPE could significantly reduce the pathological symptoms and biochemical parameters of BPH and adjust the level of the marker metabolites. The mechanism may be related to the inhibition of inflammation and alleviation of lipid metabolism disorders.
