Identification and Quantification of Adulterated Beef by Liquid Chromatography Tandem Mass Spectrometry
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Abstract:
Differential proteomics was used as the basis for theoretical study, while high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was employed to identify and quantify beef adulterated with pork and chicken. First, polypeptides relatively specific to beef, pork, and chicken were identified using a high-resolution mass spectrometer (nLC-QE), followed by product ion scan to determine the specific ion pairs in the three types of meats. These specific ion pairs were then detected in the beef adulterated with pork or chicken using HPLC-MS/MS in multiple reaction monitoring (MRM) mode, and a qualitative study was carried out using nine, eight, and seven quantitative peptides from beef, pork, and chicken, respectively. The results were compared with those obtained from polymerase chain reaction (PCR), and the results were found to be consistent with each other. Chicken and pork were blended in beef in proportions of 0.5, 1.0, 5.0, 10.0, and 25.0%, and three quantitative peptides specific to each type of meat were selected to perform the quantitative analysis. The linear correlation coefficient for each quantitative ion pair was more than 0.99, and the limit of quantitation was 0.5% blending. The results showed that the HPLC-MS/MS method can quickly and efficiently identify pork and chicken in beef, and can be used for accurate quantitative analysis.
