ABTS Radical Scavenging Activity of Theaflavins Combined with Fungal Polysaccharides
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Abstract:
The 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activities of theaflavins (TFs) alone and combined with Auricularia auricular polysaccharides (AAP) and Ganoderma lucidum spore polysaccharides (GLP) were compared in this paper. The results of ABTS radical scavenging experiments were analyzed using the Chou-Talalay combination index (CI), and the scavenging effects, combination index (CI), and dose-reduction index (DRI) of TFs, AAP, and GLP, alone and in combination, were evaluated. The half maximal inhibitory concentration (IC50) values of TFs, AAP, and GLP were 20.60 μg/mL, 2.49 mg/mL, and 0.93 mg/mL, respectively, while the IC50 values of a combination of TFs and AAP (mass ratio 1:3) and a combination of TFs and GLP (mass ratio 1:3) were 74.04 μg/mL and 58.92 μg/mL, respectively. CI analysis indicated that with the clearance rate in the range of 5~97%, the CI values of TFs with GLP were < 1 and exhibited a downward trend, suggesting a synergistic effect between TFs and GLP. DRI analysis demonstrated that with the clearance rate in the range of 5~97%, the DRI values of TFs with GLP were greater than 1. Inconsistent results were obtained in the above analyses for a combination of TFs and APP. Therefore, there was a synergistic effect between TFs and GLP for scavenging ABTS free radicals, but no similar effect was found between TFs and APP.
