Therapeutic Effect of Pu-erh Tea Theabrownins of Different Molecular Weights on Hyperlipidemic Rats
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Abstract:
The therapeutic effect of Pu-erh tea theabrownins (TB) with different molecular weights to thehyperlipidemia in a Sprague-Dawley (SD) rat model was explored. After 45 d of treatment, the levels of serum total cholesterol (TC), triglycerides (TG), and low-density lipoprotein (LDL) of rats in the TB1 and crude TB (RTB) groups were significantly lower than those of the hyperlipidemia model group (p<0.01). Furthermore, the level of high-density lipoprotein (HDL) in these rats was significantly higher than that of hyperlipidemia model group (p<0.01); their TG and HDL levels were comparable to those of control rats. Enzymatic analysis showed that TB1 and RTB had no significant effect on the activity of hormone-sensitive lipase (HSL) in liver tissue. However, HSL activities in the epididymal and mesenteric tissues were significantly higher than those of the hyperlipidemia model group (p<0.01), and these activities were not significantly different (p>0.01) than those of the normal control. Furthermore, TB1 and RTB were able to significantly inhibit the activity of acetyl-CoA carboxylase (ACC) (p<0.01) and fatty acid synthase (FAS) (p<0.05) in the fatty tissue of hyperlipidemic rats. The results indicated that RTB and TB1 can increase HSL activity in the adipose tissue of hyperlipidemic rats to accelerate lipid metabolism and reduce fatty acid synthesis by inhibiting the activities of ACC and FAS. Western blot analysis demonstrated that TB1 and RTB could upregulate HSL protein expression and downregulate ACC1 protein expression. Liver biopsy showed that TB1 and RTB could also effectively reduce the rate and extent of hepatic steatosis in the hyperlipidemic rats. Together, the results showed that TB1 was the main active component of RTB and had certain therapeutic effects in hyperlipidemic rats.
