Preparation of Chitosan/Cellulose Sulfate Composite Beads and Their Bovine Serum Albumin Adsorption Behavior
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Composite beads of chitosan and cellulose sulfate (CHT-CS) were prepared by squeezing with ionic liquid [Emim]Ac as the solvent. The preparation conditions were optimized, and the CHT-CS beads were characterized by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), and X-ray diffraction (XRD) measurement. The adsorption capacity of CHT-CS beads for bovine serum album (BSA) was evaluated, and the conditions affecting BSA adsorption were investigated. The results indicated that the optimum conditions for CHT-CS bead preparation were a cellulose sulfate substitution degree of 0.58, a composite raw material concentration of 5% (m/V), and a CS/CHT ratio of 1:1. A complex interaction between cellulose sulfate and chitosan occurred during the preparation of the composite beads, providing structural stability with many gaps and holes on the surface and in the interior of the beads. The maximum BSA adsorption capacity of the composite beads (157 mg/g) was achieved when the initial concentration of BSA was 1 mg/mL and the pH was 5. The adsorption capacity increased with the amount of CHT-CS beads, within a certain range. These results may potentially provide a reference for the separation and immobilization of BSA.
