Fabrication of Gold Magnetic Nanoparticles and Their Application for Rapid Detection of Aflatoxin B1 Using Surface-enhanced Raman Spectroscopy
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Abstract:
To introduce -NH2 and -SH to the surface of Fe3O4/SiO2 nanoparticles, Fe3O4 nanoparticles were first modified with tetraethoxylsilane to form Fe3O4/SiO2 nanoparticles, followed by the addition of 3-thiolpropyltriethxoysilane and 3-aminopropyltriethxoysilane. Au nanoparticles were then assembled on the surface of Fe3O4/SiO2 via the electrostatic adsorption of -NH2 and the effect of the Au-S bond to produce core-shell Fe3O4/SiO2/Au gold magnetic nanoparticles. These magnetic nanoparticles were characterized by a variety of techniques such as transmission electron microscopy (TEM), energy dispersive X-ray spectrometer (EDX) and ultraviolet spectrophotometer (UV-vis). Using Fe3O4/SiO2/Au gold magnetic nanoparticles as a Raman active substrate, aflatoxin B1 (AFB1) was directly and rapidly detected by surface-enhanced Raman spectrometry. In the absence of external magnetic concentration of gold magnetic nanoparticles, the detection limit of AFB1 was higher than 10.0 μg/mL, while in the presence of external magnetic concentration, the detection limit was decreased 100-fold (≤0.1 μg/mL) with a linear detection range of 0.1~10.0 μg/mL. The recovery rate of samples was 84.35~91.98% and the relative standard deviation was 4.88~9.90%.
