Effects of Electron Beam Irradiation on the Myofibrillar Protein Structure and Gel Properties of Collichthys lucidus Surimi
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Abstract:
To determine the optimal radiation dose, the effects of electron beam irradiation (0?9 kGy) on the myofibrillar protein structure and gel properties of Collichthys lucidus surimi were evaluated. The results showed that at radiation doses of 7 kGy and 9 kGy, the total sulfhydryl group and active sulfhydryl group content decreased significantly, compared to those treated with lower doses (≤5 kGy). With increasing doses of radiation, the activity of calcium ATPase decreased significantly, while the surface hydrophobicity of surimi myofibrillar proteins increased initially and then decreased. The highest surface hydrophobicity was reached at 5 kGy. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and differential scanning calorimetry (DSC) revealed that the thermal stability of myosin heavy chains (MHC), myosin, and actin decreased significantly with the increase in radiation doses, especially in the 7 kGy and 9 kGy groups. The gel strength, whiteness, and expressible water content of surimi gel were the highest when 5 kGy of electron beam irradiation was performed. Compared to the high-dose groups (≥7 kGy), the low-dose groups did not significantly affect the structure of surimi myofibrillar proteins. Thus, irradiation with 5 kGy could effectively improve the gel properties of Collichthys lucidus surimi.
