Green Biosynthesis of Silver Nanoparticles Using an Endophytic Fungus
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Abstract:
DO-1, an endophytic fungus, was isolated from Dendrobium candidum and identified as Fusarium graminearum. The fungal biomass was immersed in distilled water, and the filtrate solution containing the metabolites was obtained. Silver nanoparticles (AgNPs) with antimicrobial activity were biosynthesized by mixing the filtrate solution with silver nitrate. The plasma absorption peak of UV-visible spectroscopy and antibacterial activity were measured to assess the effects of light, temperature, and pH on AgNP biosynthesis. Darkness and suitable high temperatures were beneficial to the biosynthesis and antibacterial activity of AgNPs and absorbance at 440~450 nm increased with increasing pH. The highest antibacterial activity was observed at pH 7~8, and it decreased at higher pH levels. AgNPs synthesized from F. graminearum DO-1 filtrate solution and 1 mM silver nitrate at pH 7 and 80 ℃ for 3 h in the darkness, were spherical shaped with the diameter range of 2~50 nm. The method of AgNPs biosynthesis used herein is simple, uses mild conditions, and is eco-friendly.
